{"status":"OK","msg":"Details loaded!","presentation":{"id":56,"title":" BRAIN MULTIELEMENT ANALYSIS ON ALZHEIMER'S DISEASE (AD) INDUCED BY BETA AMYLOID OLIGOMERS IN SWISS MICE ","abstract":"Alzheimer disease (AD) is an insidious, progressive and irreversible brain disorder. Its incidence is highest in people aged over 65 [1]. The cause of AD is not fully understood. The pathological features of AD include \u03b2-amyloid (A\u03b2) plaques, intracellular neurofibrillary tangles, loss of neurons and synapses, and activation of glia cells. Recently, several groups have raised the \u201cmetal hypothesis\u201d of AD [2]. This hypothesis is mainly based upon experimental results. First, several metal ions, such as zinc, iron and copper, are all increased in the ageing brain. It has been reported that the content of metal ions in the brain of AD patients is 3-7 times that of normal people and that the metal ions in brain homeostasis may result in the development of AD [3]. Second, these metal ions can interact with A\u03b2, resulting in the promotion of A\u03b2 deposition and plaque formation. Total reflection X-ray fluorescence analysis (TXRF) is a multielement analytical technique, which is capable of yielding quantitative information on the elemental composition of a variety of materials. This method can be used for elemental trace analysis [4]. In this work, three groups were studied: control, AD10 and AD100. The groups AD10 and AD100 were given a single intracerebroventricular injection of 10 pmol and 100 pmol of oligomers of \u03b2-amyloid peptide respectively to be induced AD. Seven days after the injection was carried out craniectomy and subsequently evaluated five brain compartments: frontal cortex, temporal cortex, hypothalamus, hippocampus and cerebellum. All samples were submitted to acid digestion (HNO3 + H2O2). After that, they were diluted with Milli-Q water and added an internal standard (Ga).The TXRF measurements were performed at the X-Ray Fluorescence Beamline at Brazilian National Synchrotron Light Laboratory (LNLS), in Campinas, S\u00e3o Paulo using a monochromatic beam (11.5 keV) for the excitation and an Silicon drift detector with energy resolution of 133 eV at 5.9 keV. It was possible to determine the concentrations of the following elements: P, S, K, Ca, Fe, Cu and Zn.","keywords":"Alzheimer's Disease; Multielement Analysis; Fluorescence; Synchrotron","status":"active","sarx_st":"accepted","user_id":115,"created_at":"2016-05-11 15:39:51","updated_at":"2016-08-20 17:04:21","filename":"Abstract_Danielle Almeida.doc","mode":"oral","sarx_code":"04_O-XRF","poster_group":0,"file":"5dad497794fe3ed186d9314f72427d54.doc","author":"Danielle Santos de Almeida ","affiliation":"Doutorado \/<\/b> Universidade Federal do Rio de Janeiro \/<\/b> Brasil","user":{"id":115,"name":"Danielle Santos de Almeida ","degree":"Doutorado","email":"dani.almeida84@gmail.com","username":"dani.almeida84@gmail.com","confirmed":0,"confirmation_code":"WEK5tbioIOJGpqWqKhtYqfKPpAsTvF","created_at":"2016-05-04 20:07:30","updated_at":"2016-09-13 20:35:42","sarx":1,"affiliation":"Universidade Federal do Rio de Janeiro","address":"Rua Buarque de Macedo, 14\/405","zipcode":"22220030","state":"Rio de Janeiro","phone":"984099903","fax":null,"city":"Rio de Janerio","country":"Brasil","status":"active","is_admin":0,"category":"student","school":1,"icdd":1}}}