{"status":"OK","msg":"Details loaded!","presentation":{"id":81,"title":"ZINC INTENSITY IN TUMOR HUMAN CELLS PROSTATIC AND NORMAL CELLS USING SYNCHROTRON X-RAY MICROFLUORESCENCE ","abstract":"Cancer is a worldwide public health problem, and its incidence in the world grew by 20% in the last decade. Zinc, an essential trace element, is involved in many cellular processes. The main function of the prostate is the high production and secretion of citrate which is possible due to the unique accumulation of zinc by prostatic epithelial cells. In relation to prostate cancer, it can inhibit the growth of tumor cells, due to the stop of cell cycle and \/ or induction of apoptosis. The X-ray microfluorescence technique (\u03bcXRF) is a tool for modern analysis of elemental composition of different samples with high sensitivity and negligible damage to the sample. This study investigated the behavior of cells in spheroids of human prostate cells, tumor cell line (DU145) and normal cell line (RWPE- 1), after supplementation with zinc chloride by 24 hours and 48 hours using synchrotron X-ray microfluorescence (\u00b5SRXRF) and analyzed cell viability through colorimetric cytotoxicity assay (MTT). The measurements were performed with a standard geometry of 45\u00ba of incidence, excited by a white beam using a pixel of 25 \u03bcm and a time of 300 ms\/pixel at the XRF beamline at the Synchrotron Light National Laboratory (Campinas, Brazil). The results by \u00b5SRXRF showed that Zn intensity decreased in tumor cells despite supplementation and the treatment time, while in normal cells that intensity increases with supplementation and the treatment time. MTT assay's results were not significant, which indicates that the cell viability was not changed, regardless of treatment in both cell types tested, so the cells are viable.","keywords":"X-Ray; Microfluorescence; Synchrotron; cancer; zinc","status":"active","sarx_st":"accepted","user_id":144,"created_at":"2016-07-05 17:17:28","updated_at":"2016-08-20 12:24:54","filename":"Abstract _SARX2016__Karolynne.pdf","mode":"oral","sarx_code":"16_O-XRF","poster_group":null,"file":"b1f253af5266a5008c853c8906fd0cd5.pdf","author":"Karolynne de Araujo Marins Jos\u00e9","affiliation":"Estudante de Doutorado \/<\/b> COPPE\/UFRJ \/<\/b> Brasil","user":{"id":144,"name":"Karolynne de Araujo Marins Jos\u00e9","degree":"Estudante de Doutorado","email":"karolynnearaujo@gmail.com","username":"karolynnearaujo@gmail.com","confirmed":1,"confirmation_code":null,"created_at":"2016-05-09 17:38:01","updated_at":"2016-07-05 17:23:04","sarx":1,"affiliation":"COPPE\/UFRJ","address":"Estrada do Barro Vermelho, 600","zipcode":"20540500","state":"Rio de Janeiro","phone":"21988414290","fax":null,"city":"Rio de Janeiro","country":"Brasil","status":"active","is_admin":0,"category":"student","school":1,"icdd":0}}}